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Thermo Scientific™

GeneBLAzer™ ERRα-UAS-bla HEK 293T Cells

Catalog number: K1702
Thermo Scientific™

GeneBLAzer™ ERRα-UAS-bla HEK 293T Cells

Catalog number: K1702
Catalog Number
K1702
Unit Size
2 x 106 cells
Price (USD)
8,140.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K17022 x 106 cells
8,140.00
Your Price:
***
Product Overview
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ERR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Estrogen related receptor alpha (ERR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ERR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ERR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of XCT790, an inverse agonist. In addition, ERR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists
L165, 041, GW 1929, GW 7647, GW 0742
Antagonists
T0070907, GW 9662, GW 6471
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
Druggable Target
Nuclear Receptors
Primary Agonist
Rosiglitazone
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
System Type
GeneBLAzer™
Target Entry
ESRRA, NR3B1, ERR1, ERR alpha
Type
HEK 293T Cell

Contents & Storage

GeneBLAzer™ Nuclear Receptor Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.

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