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GeneBLAzer® M1-NFAT-bla Jurkat cells contain the human Acetylcholine (muscarinic) subtype 1 receptor (M1) stably integrated into the CellSensor® NFAT-bla Jurkat cell line. CellSensor® NFAT-bla Jurkat cells (Cat. no. K1077) contain a beta-lactamase (bla) reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.
M1-NFAT-bla Jurkat cells are functionally validated for Z'-factor and EC50 concentrations of carbachol. In addition, M1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
In the brain, M1 activation mediates 'slow' neuronal excitability. Cortical and hippocampal muscarinic receptors are thought to be important in the attentional aspects of cognition. The predominant receptor subtypes in these brain areas are M1, M3, and M4. Therefore, M1 is a potential target for cognition, Alzheimer's, dementia, and schizophrenia. Studies on knock-out mouse models of M1 are also beginning to reveal potential functions of the receptor. Additional information on the muscarinic receptors can be found in reviews.
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For Research Use Only. Not for use in diagnostic procedures.
Cell-based beta lactamase reporter gene
For Use With (Application)
Single Cell Clone Origin
Contents & Storage
Contents: K1710 GeneBLAzer™ M1-NFAT-bla Jurkat cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ M1-NFAT-bla Jurkat cells • GeneBLAzer™ M1-NFAT-bla Jurkat Assay Protocol • Certificate of Analysis
Storage: The GeneBLAzer™ M1-NFAT-bla Jurkat cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.