Thermo Scientific™

GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK 293T Cells

Catalog number: K1713
Thermo Scientific™

GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK 293T Cells

Catalog number: K1713
Catalog Number
K1713
Unit Size
2 × 10^6 cells
Price (USD)
Price: 9,190.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K17132 × 10^6 cells
Price: 9,190.00
Your Price:
-
Product Overview
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GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells contain the human Endothelial Differentiation Receptor 3 (EDG3) stably integrated into the GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cell line. GeneBLAzer™ Gα15-NFAT-bla HEK293T cells (Cat. no. K1539) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.

The GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells are functionally validated for Z' and EC50 concentrations of S1P. In addition, GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

EDG-3/S1P-3(Sphingosine-1-Phosphate-3) is a Gq/Gi/Go/Gα13/Gα12 coupled GPCR. EDG-3 has been shown to be responsible for bradycardia that has been induced in clinical trials as a side effect of the immunosuppressive drug FTY720 that targets EDG-1. EDG-3 has also been shown to induce vasodilation in response to sphingosylphosphorylcholine (SPC), sphingosine-1-phosphate (S1P), and lysosulfatide (LSF). The EDG-3 Receptor has also been shown to play a cooperative or redundant role in angiogenesis with EDG-1.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NM_005226.2
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
EDG3
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
EDG3
Cell Line
293T (HEK)
System Type
GeneBLAzer™
Druggable Target
GPCRs

Contents & Storage

Contents:
K1713 GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells
• GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293TAssay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.