During checkout, this product may be reported as "Out of Stock" with an Estimated Availability date. The Actual Availability date for this product may be earlier. Send inquiries regarding Actual Availability date, including the catalog number, to discoverysciences@lifetech.com.

GeneBLAzer® EDG3-Gα15-NFAT-bla HEK 293T Cells

Catalog number: K1713

 Related applications: Target & Lead Identification & Validation

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells contain the human Endothelial Differentiation Receptor 3 (EDG3) stably integrated into the GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cell line. GeneBLAzer® Gα15-NFAT-bla HEK293T cells (Cat. no. K1539) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.

The GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells are functionally validated for Z' and EC50 concentrations of S1P. In addition, GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

EDG-3/S1P-3(Sphingosine-1-Phosphate-3) is a Gq/Gi/Go/Gα13/Gα12 coupled GPCR. EDG-3 has been shown to be responsible for bradycardia that has been induced in clinical trials as a side effect of the immunosuppressive drug FTY720 that targets EDG-1. EDG-3 has also been shown to induce vasodilation in response to sphingosylphosphorylcholine (SPC), sphingosine-1-phosphate (S1P), and lysosulfatide (LSF). The EDG-3 Receptor has also been shown to play a cooperative or redundant role in angiogenesis with EDG-1.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell Line: 293T (HEK)
Technique: FRET
Cell State: Dividing Cells
Gene Symbol: EDG3
Product Size: 2 x 106 cells
Accession Number: NM_005226.2
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Contents & storage

Contents:
K1713 GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells
• GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293TAssay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® EDG3-Gα15-NFAT-bla HEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Documents

Manuals & protocols