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GeneBLAzer® OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cells

Catalog number: K1714

 Related applications: Target & Lead Identification & Validation

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Description

The GeneBLAzer® OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cell Line contains the human opioid/opiate receptor-like-1 receptor (OPRL1) stably integrated into the GeneBLAzer® Gqi5-NFAT-bla FreeStyle™ 293F Cell Line. GeneBLAzer® Gqi5-NFAT-bla FreeStyle™ 293F cells (Cat. no. K1539) contain the chimeric Gqi5 G protein and the beta-lactamase reporter gene under control of the nuclear factor of activated T cells (NFAT) response element.

OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and functionally validated for Z-factor and EC50 concentrations of orphanin FQ/nociceptin. These data are found in the Validation & Assay Performance Summary.

Orphanin FQ/nociceptin and OPRL1 have been implicated in various physiological functions. The most defined role is their involvement in the mediation of pain in the central nervous system and their possible roles in opiate tolerance, opiate dependence and withdrawal, and the ability to cope with stress and anxiety. Through the use of antagonists, antisense oligos, and transgenic knockout mutants, OPRL1 has been implicated in additional systems including learning, memory, and reproduction.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell Line: FreeStyle™ 293-F Cells
Technique: FRET
Cell State: Dividing Cells
Gene Symbol: OPRL1
Product Size: 2 x 106 cells
Accession Number: NM_000913
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Contents & storage

Contents:
K1714 GeneBLAzer® OPRL1-Gqi5-NFAT-bla Freestyle 293F cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® OPRL1-Gqi5-NFAT-bla Freestyle 293F cells
• GeneBLAzer® OPRL1-Gqi5-NFAT-bla Freestyle 293F Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® OPRL1-Gqi5-NFAT-bla Freestyle 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Documents

Manuals & protocols