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The GeneBLAzer® OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cell Line contains the human opioid/opiate receptor-like-1 receptor (OPRL1) stably integrated into the GeneBLAzer® Gqi5-NFAT-bla FreeStyle™ 293F Cell Line. GeneBLAzer® Gqi5-NFAT-bla FreeStyle™ 293F cells (Cat. no. K1539) contain the chimeric Gqi5 G protein and the beta-lactamase reporter gene under control of the nuclear factor of activated T cells (NFAT) response element.
OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and functionally validated for Z-factor and EC50 concentrations of orphanin FQ/nociceptin. These data are found in the Validation & Assay Performance Summary.
Orphanin FQ/nociceptin and OPRL1 have been implicated in various physiological functions. The most defined role is their involvement in the mediation of pain in the central nervous system and their possible roles in opiate tolerance, opiate dependence and withdrawal, and the ability to cope with stress and anxiety. Through the use of antagonists, antisense oligos, and transgenic knockout mutants, OPRL1 has been implicated in additional systems including learning, memory, and reproduction.
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For Research Use Only. Not for use in diagnostic procedures.
Cell-based beta lactamase reporter gene
For Use With (Application)
Single Cell Clone Origin
FreeStyle™ 293-F Cells
Contents & Storage
Contents: K1714 GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F cells • GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F Assay Protocol • Certificate of Analysis
Storage: The GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.