GeneBLAzer™ M3-NFAT-bla CHO-K1 cells contain the human Acetylcholine (Muscarinic) Receptor 3 (M3) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.
The GeneBLAzer™ M3-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Carbachol. In addition, GeneBLAzer™ M3-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
The M3 receptor is localized in smooth muscle, exocrine glands, and the central nervous system. M3 has been implicated in autoimmune and neurological disorders.
For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_000740.2
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
M3
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
M3
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCRs
Contents & Storage
Contents: K1716 GeneBLAzer™ M3-NFAT-bla CHO-K1 cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ M3-NFAT-bla CHO-K1 cells • GeneBLAzer™ M3-NFAT-bla CHO-K1 Assay Protocol • Certificate of Analysis
Storage: The GeneBLAzer™ M3-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.