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Thermo Scientific™

GeneBLAzer™ BDKRB1-NFAT-bla CHO-K1 Cells

Catalog number:  K1734

Related applications:

Pharma & Biopharma

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Description

GeneBLAzer® BDKRB1-NFAT-bla CHO-K1 cells contain the human Bradykinin Receptor 1 (BDKBR1) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer® BDKRB1-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of [Lys-des-Arg9]-Bradykinin. In addition, GeneBLAzer® BDKRB1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The Bradykinin-BDKRB1 receptor has been implicated in inflammation and pain. Preclinical evidence demonstrates that interruption of BDKRB1 receptor function causes analgesia under a variety of conditions and has been well established using selective antagonists and genetically modified mice.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_000710
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: BDKRB1
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: BDKRB1
Technique: FRET

Contents & storage

Contents:
K1734 GeneBLAzer® BDKRB1-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® BDKRB1-NFAT-bla CHO-K1 cells
• GeneBLAzer® BDKRB1-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® BDKRB1-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.