The Tango™ EDG6-bla U2OS cells contain the human Endothelial Differentiation, Lysophosphatidic Acid G-protein-coupled Receptor, 6 (EDG6) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ EDG6-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ EDG6-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.
Cell-based beta-arrestin recruitment
Tail from GRPR GPCR
Pool of Stable Cells
Contents & Storage
K1757 Tango™ EDG6-bla U2OS DA Assay Kit Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • Tango™ EDG6-bla U2OS DA cells • LiveBLAzer™-FRET B⁄G Loading Kit, 70µg • Solution D • Tango™ EDG6-bla U2OS DA Assay Protocol • LiveBLAzer™-FRET B⁄G Loading Protocol • Certificate of Analysis
Storage: The Tango™ EDG6-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.