Thermo Scientific™

Tango™ AGTRL1-bla U2OS DA Assay Kit

Catalog number: K1762
Thermo Scientific™

Tango™ AGTRL1-bla U2OS DA Assay Kit

Catalog number: K1762
Catalog Number
K1762
Unit Size
1 kit
Price (USD)
902.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K17621 kit
902.00
Your Price:
***
Product Overview
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The Tango™ AGTRL1-bla U2OS DA cells contain the human Angtiotension-Like Receptor 1 (AGTRL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ AGTRL1-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ AGTRL1-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

Accession Number
NM_005161
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Division-Arrested Cells
Cytoplasmic Tail
Native Tail
Detection Method
Fluorescent
Gene Symbol
AGTRL1
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Protein Family
GPCR (G-Protein Coupled Receptors)
Readout
End Point
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
AGTRL1
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCRs

Contents & Storage

K1769 Tango™ AGTRL1-bla U2OS DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• Tango™ AGTRL1-bla U2OS DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• Tango™ AGTRL1-bla U2OS DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis
The Tango™ AGTRL1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

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