Easy-DNA™ gDNA Purification Kit

Catalog number: K180001

Invitrogen™  Related applications: DNA Extraction

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The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Other samples tested include mouse tails, plant leaves, yeast, and E. coli cells. These samples yield high-quality DNA with an average size between 100 kb and 200 kb, which is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications. Advantages of the Easy-DNA™ Kit include:

Reliability—high-quality genomic DNA from large or small samples of cells, tissue, plant, yeast, E. coli, blood, and even hair follicles
Simplicity—for most sample types, only 4 steps, typically requiring less than 90 minutes are needed to isolate high-quality DNA
Versatility—one kit is supported by optimized protocols for a variety of cell and tissue types

Simple workflow, quality results
Optimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Fungi, Bacteria, Yeast, Tissue, Plant Samples, Cells
Isolation Technology: Organic Extraction
Downstream Application: PCR, Southern Blotting
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Size: 1 kit

Contents & storage

The Easy-DNA™ Kit contains enough reagents for 15–200 samples, depending on sample size and type.

This kit contains:
• 55 ml Solution A (Lysis Solution)
• 25 ml Solution B (Precipitation Solution)
• 100 ml TE Buffer
• 750 µl Mussel Glycogen (2 mg/ml in sterile water)
• 750 µl RNase (2 mg/ml in sterile water)
• 750 µl Protein Degrader (5 mg/ml in sterile water)

Store kit at room temperature. For long-term storage, Mussel Glycogen, RNase, and Protein Degrader may be stored at -20°C.


Manuals & protocols

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