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Thermo Scientific™

GeneBLAzer™ MCHR2-NFAT-bla CHO-K1 Cells

Catalog number:  K1852

Related applications:

Pharma & Biopharma

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Description

Tango™ MCHR2-bla U2OS cells contain the human Melanin-concentrating Hormone 2 (GPR145) (MCHR2) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ MCHR2-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of Melanin Concentrating Hormone (6-17). In addition, Tango™ MCHR2-bla U2OS cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_032503.2
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®
Readout: End Point
System Type: GeneBLAzer™
Target Entry: GPR145, MCHR2
Technique: FRET

Contents & storage

GeneBLAzer® MCHR2-NFAT-bla CHO-K1 cells are shipped on dry ice, 8 x 10^6 cells⁄vial. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.