During checkout, this product may be reported as "Out of Stock" with an Estimated Availability date. The Actual Availability date for this product may be earlier. Send inquiries regarding Actual Availability date, including the catalog number, to discoverysciences@lifetech.com.
Thermo Scientific™

Tango™ HTR1A-bla U2OS Cells

Catalog number:  K1857

Related applications:

Pharma & Biopharma

Back to top

Description

Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z’ factor and EC50 concentrations of 5-CT (Figure 1). In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_000524
Assay Entry: Cell-based beta-arrestin recruitment
Cell Line: U2OS
Cell State: Dividing Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: HTR1A
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Population Status: Single Cell Clone Origin
Product Line: Tango™
Readout: End Point
System Type: Tango™
Target Entry: 5HT1A, HTR1A
Technique: FRET

Contents & storage

K1857 Tango™ HTR1A-UAS-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• Tango™ HTR1A-UAS-bla CHO-K1 cells
• Tango™ HTR1A-UAS-bla CHO-K1 Assay Protocol
• Certificate of Analysis
The Tango™ HTR1A-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.