The PureLink HQ Mini Plasmid Purification Kit is designedfor the isolation of high-quality plasmid DNA that is suitablefor restriction enzyme digestion, PCR, sequencing, bacterialcell transformation, and mammalian cell transfection.Using the kit, plasmid DNA can be isolated from varyingamounts of bacterial cells.
The PureLink HQ Mini Plasmid Purification Kit has thefollowing advantages: • Higher spin column capacity for plasmid DNA ascompared to other commercially available plasmidpurification systems • Designed to isolate high-quality plasmid DNA in less thanan hour • Minimal genomic DNA contamination of the purifiedsample • Reliable performance of the purified DNA in downstreamapplications
Process overview Bacterial cells are pelleted, resuspended, and lysed. Thelysate is then neutralized and conditions are adjusted forsubsequent binding. After clarification by centrifugation, thelysate is processed through the PureLink spin column. TheDNA binds to the silica-based membrane in the column, andimpurities are removed by a single wash step. The DNA isthen eluted in Elution Buffer or water.
For Research Use Only. Not for use in diagnostic procedures.
The PureLink HQ Mini Plasmid DNA Purification Kit includes sufficient PureLink columns, collection tubes, elution tubes, and reagents for 100 purifications. Store components at room temperature. Store Resuspension Buffer after addition of RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.