Invitrogen™

PureLink™ HiPure Plasmid Maxiprep Kit

Catalog number:  K210006

Related applications:

DNA Extraction

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Description

The PureLink® HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include:

Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
High-purity, low-endotoxin product—pure enough for mammalian cell transfection
Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification
The PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.

Contaminant-free DNA
Unlike cesium chloride gradient protocols, PureLink® HiPure Plasmid Purification Systems do not use organic solvents, ethidium bromide, or cesium chloride, all of which are troublesome to work with and to dispose of. Typically, plasmid DNA prepared using the PureLink® HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications. Endotoxin levels are typically within 0.1–1 EU/µg, making this plasmid DNA ideal for mammalian cell transfection.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: BAC DNA, Plasmid DNA
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Purification Target: BAC DNA, Plasmid DNA
Sample Type: Bacterial Culture
Includes: 100mL Resuspension buffer (R3), 550μL RNase A, 100mL lysis buffer (L7), 100mL precipitation buffer (N3), 300mL equilibration buffer (EQ1), 2 x 300mL wash buffer (W8), 200mL elution buffer (E4), 30mL TE buffer, 10 HiPure columns, 3 Column holders
Isolation Technology: Anion Exchange Resin with Lysate Clearing Filter
No. of Reactions: 10 Preps
Quantity: 10 columns
Yield: 1000 µg
Elution Volume Max: 15 ml
Elution Volume Min: 15 ml
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Prep Scale: > 200 µg (Large-Scale) Plasmid DNA
Purity or Quality Grade: Transfection Grade
Purification Time: 2 hrs
Shipping Condition: Room Temperature
System Type: PureLink™
Product Line: PureLink™

Contents & storage

• 100 ml Resuspension Buffer (R3)
• 550 µl RNase A
• 100 ml Lysis Buffer (L7)
• 100 ml Precipitation Buffer (N3)
• 300 ml Equilibration Buffer (EQ1)
• 2 × 300 ml Wash Buffer (W8)
• 200 ml Elution Buffer (E4)
• 30 ml TE Buffer
• 10 HiPure Columns
• 3 Column Holders

Store all components at room temperature.