This product, K210009, will be discontinued on September 2016. An identical product, PureLink™ HiPure Expi Plasmid Megaprep Kit, Cat # K210009XP that has better performance, same protocol, and same price, is available as an alternative.

PureLink® HiPure Plasmid Gigaprep Kit

Catalog number: K210009

Invitrogen™  Related applications: DNA Extraction

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


• Fast, simple protocols - Purify your plasmid DNA in less than 2 hours
• Labor-saving - No need for extra steps to remove endotoxin and other contaminants
• Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges

PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA is pure enough for transfections with no need for additional steps to remove contaminants like RNA, proteins, and endotoxin (Figure 2). Additionally, phenol, chloroform, ethidium bromide, and cesium chloride are eliminated, minimizing exposure to, and disposal of, hazardous materials.
For Research Use Only. Not for use in diagnostic procedures.


High Throughput Compatibility: High Throughput-Compatible
Sample Type (General): Bacteria
Downstream Application: PCR, Transfection, Cloning, Transformation, Nucleic Acid Labeling, Sequencing, Next-Generation Sequencing, In Vitro Transcription
Product Size: 2 preps
Final Product: Plasmid DNA
Isolation Technology: Vacuum Filter Column, Anion Exchange Resin
Sample Type (Specific): Bacterial Culture
Shipping Condition: Room Temperature

Contents & storage

All components of the PureLink® HiPure Plasmid DNA Purification Kits are shipped at room temperature. Upon receipt, store all components at room temperature.


RNase A
(20 mg/ml in R3)
Lysis Buffer (L7)
Precipitation Buffer (N3)
Equilibration Buffer (EQ1)
Wash Buffer (W8)
Elution Buffer (E4)
TE Buffer (TE)


Manuals & protocols

Recommended products