Invitrogen™

pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Catalog number: K250020
Invitrogen™

pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Catalog number: K250020
Catalog Number
K250020
also known as K2500-20
Unit Size
20 reactions
Price (USD)
532.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K250020
also known as K2500-20
20 reactions
532.00
Your Price:
***
Product Overview
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The pCR™8/GW/TOPO™ TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway™ recombination cloning vector platform. Our pCR™8 TOPO™ TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector.

With our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway™ recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.

The pCR™8/GW/TOPO™ TA Cloning Kit offers:

att sites for rapid recombination into a variety of Gateway™ destination vectors
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoRI sites flanking the PCR product insertion site for easy excision of inserts

This kit includes One Shot™ TOP10 Chemically Competent E. coli.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
TA Cloning Kit
Cloning Method
TOPO™ with Gateway™
Vector
pCR
Product Line
One Shot™

Contents & Storage

The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

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