Search

  • Contáctenos
  • Orden Rápida
Zero Blunt™ PCR Cloning Kit, without competent cells, 40 Reactions - FAQs

Zero Blunt™ PCR Cloning Kit, without competent cells, 40 Reactions - FAQs

View additional product information for Zero Blunt™ PCR Cloning Kit, without competent cells - FAQs (K275040, K275020)

2 product FAQs found

Which of the following products will usually give the best cloning efficiency in terms of positive clones and number of colonies: Zero Blunt PCR, Zero Blunt PCR-TOPO, or Zero Blunt PCR-TOPO for Sequencing?

Typically 200-300 colonies are obtained for the TOPO kits with 100% inserts (20/20). For the Zero Blunt kit that uses standard ligase, typically 600-1000 colonies are obtained with 95-100% inserts.

Is it possible to generate nested deletions using the TOPO Cloning Kit for Sequencing and the ZeroBlunt TOPO PCR Cloning Kits?

Yes. Both vectors (pCR4 and pCR-Blunt II) are designed to allow the creation of nested deletions for sequencing large inserts using the same sequencing primer. In addition, a comprehensive protocol is provided in the TOPO manual for these kits.