MagJET NGS Cleanup and Size Selection Kit
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MagJET NGS Cleanup and Size Selection Kit
Thermo Scientific™

MagJET NGS Cleanup and Size Selection Kit

Thermo Scientific MagJET NGS Cleanup and Size Selection kit is designed for efficient and robust DNA fragment library cleanup andRead more
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Catalog NumberQuantity
K282810 Preps
K28224 x 96 Preps
K28211 x 96 Preps
K282310 x 96 Preps
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Catalog number K2828
Price (USD)/ 10 preps
36.33
-
Add to cart
Quantity:
10 Preps
Price (USD)/ 10 preps
36.33
Add to cart
MagJET NGS Cleanup and Size Selection Kit
Catalog numberK2828
Price (USD)/ 10 preps
36.33
-
Add to cart

Thermo Scientific MagJET NGS Cleanup and Size Selection kit is designed for efficient and robust DNA fragment library cleanup and size-selection from a variety of enzymatic reaction mixtures including PCR, ligation, adapter addition and DNA end-repair reaction mixes. The kit utilizes high-capacity MagJET paramagnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. The purified DNA fragments are free of any next-generation sequencing workflow inhibiting components, such as sequencing adapters, primer dimers, unincorporated nucleotides, enzymes or salts, and are ready-to-use in downstream NGS applications. No prior DNA fragment library purification is required before the cleanup and size-selection procedures.

The kit is suitable for cleanup or size selection of 5 ng to 5 μg of DNA fragment library. MagJET cleanup and adapter removal protocols can easily be automated for higher throughput on automatic platforms such as Thermo Scientific KingFisher Flex and Thermo Scientific KingFisher Duo.

Highlights
Robust—reliable cleanup and size selection from different reaction mixtures and buffers
Flexible—user-defined size-selection of exact DNA fragment range
NGS-grade purity—complete removal of NGS adapters, primers, nucleotides, enzymes and other reaction components inhibiting NGS workflow
High throughput compatible—easy to automate on the Thermo Scientific KingFisher magnetic particle processors for high-throughput sample handling

Applications
DNA fragment library cleanup and size-selection for downstream use in next generation sequencing workflows.

Includes
• MagJET Magnetic Beads
• Binding Solution
• Wash Buffer (concentrated)
• Elution Buffer
• DNA Fragment Mix

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MagJET NGS Cleanup and Size Selection Kit

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologyMagnetic Bead
Sample TypeEnzymatic Reactions (PCR, ligation, adapter addition, DNA end-repair reaction)
Elution Volume10 to 50 μL
Final Product TypeDNA
For Use With (Equipment)KingFisher™ Flex, KingFisher™ Duo Prime
For Use With (Application)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput CompatibilityHigh-throughput Compatible
Quantity10 Preps
Shipping ConditionRoom Temperature
YieldUp to 700 bp
Starting Material Amount100 μL (5 ng to 5 μg DNA fragments)
Unit Size10 preps
Contents & Storage
• 1 mL DNA Fragment Mix; -20°C
• 110 μL MagJET Magnetic Beads; 4°C
• 8 mL Binding Buffer (concentrated); room temperature
• 3 mL Wash Buffer (concentrated); room temperature
• 2 x 1.5 mL Elution Buffer; room temperature