Invitrogen™

PureLink™ PCR Micro Kit

Catalog number:  K310050

Related applications:

DNA Extraction

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Description

PureLink® PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions. These highly purified DNA fragments and amplicons provide excellent results in downstream applications such as DNA sequencing, restriction digestion, cloning, PCR, and labeling. PureLink® PCR Micro Kits provide a simple and rapid method for purifying and concentrating DNA fragments ranging in size from 100 bp-12 Kb. The unique micro-nozzle design allows up to 95% recovery of DNA fragments with as little as a 5 µl elution volume. With less than a 10% coefficient of variance in the elution volume you can be sure that no ethanol is carried over from the wash steps giving you the best chance of success in downstream applications. Removal of primers, dNTPs, enzymes, and salts exceeds 98.5%. This kit can also efficiently remove unincorporated dye-labeled nucleotides from a cDNA labeling reaction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): PCR, Sequencing, Nucleic Acid Labeling, Cloning
Purification Target: DNA from Enzymatic Reactions (e.g. PCR)
Sample Type: DNA (Restriction Enzyme-Digested), PCR Products, cDNA Labeling Reactions
Isolation Technology: Silica Spin Column
Quantity: 50 columns
Separation Range: 100 bp to 12 kb
Elution Volume Max: 20 µl
Elution Volume Min: 5 µl
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 10 min
Shipping Condition: Room Temperature
Starting Material Volume: 125 µl
Product Line: PureLink™

Contents & storage

Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.