Vivid Colors™ pcDNA™6.2/N-EmGFP-GW/TOPO® Mammalian Expression Vector

Catalog number: K36020

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis | Protein Expression

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Description

The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse it to the widely used and well characterized Fluorescent Proteins (FPs) from the jellyfish Aequorea victoria (1, 2). These powerful TOPO® cloning vectors contain the Emerald Green Fluorescent Protein (EmGFP) or the Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein (Figure 2). Both FPs have been humanized for optimal mammalian expression (3). The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors offer:
• Topoisomerase I for one-step, 5-minute TOPO® cloning of your PCR-amplified gene of interest
• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Ability to fuse EmGFP or YFP to the N- or C-terminus of your protein
• Bsd resistance marker for rapid selection of stable cell lines
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Subcellular Localization, Reporter Assays
Protein Tag or Fusion: GFP (EmGFP)
Reporter: GFP (EmGFP)
Delivery Method: Transfection
Cloning Method: TOPO® with Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pcDNA
Product Size: 20 reactions
Constitutive or Inducible System: Constitutive

Contents & storage

Each pcDNA™6.2-TOPO® Expression Vector Kit contains two boxes. The TOPO® box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO® vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO® box should be stored at -20°C. The One Shot® box contains transformation reagents including single-use 50 µl aliquots of One Shot® TOP10 chemically competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competent E. coli at -80°C. All reagents are guaranteed stable for 6 months when properly stored.

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