pcDNA™3.1/V5-His® TOPO® TA Expression Kit

Catalog number: K480001SC

Invitrogen™  Related applications: Protein Expression

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Description

The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. This kit is specifically formatted for storage in Invitrogen Supply Centers at -80°C. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression V5 epitope tag for efficient detection of recombinant proteins 6×His sequence for purification using nickel-chelating resin and detection
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Constitutive Expression
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: pcDNA
Product Size: 20 reactions
Constitutive or Inducible System: Constitutive

Contents & storage

Each pcDNA™3.1 Directional TOPO¤ Expression Kit contains two boxes. The TOPO® box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid. Store at -20°C. The One Shot® box contains single-use 50-µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols