FreeStyle™ MAX CHO Expression System

Catalog number: K900020

Invitrogen™  Related applications: Antibody & Cell Culture Production | Protein Expression | Transfection

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Description

The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

The FreeStyle™ MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures (Figure 1)
• Functional proteins (up to milligram quantities) with all post-translational modifications produced in one week
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for production scale-up

The FreeStyle™ MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one
week (Figure 2).

Contents and Storage:
Store FreeStyle™ cells in liquid nitrogen. Store FreeStyle™ MAX Reagent at +4°C. Store GIBCO® FreeStyle™ CHO Expression Medium, FreeStyle™ 293 Expression Medium, and OptiPRO™ SFM at +2-8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additonal storage information.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line: FreeStyle™ CHO-S Cells
Expression: Mammalian
Culture Type: Suspension Cell Culture
Key Function: Protein Production
Product Size: 1 kit
Classification: Animal Origin-Free, Serum-Free
Delivery Method: Transfection
Expression Mechanism: Cell-Based Expression

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Manuals & protocols

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