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Thermo Scientific™

DreamTaq™ Hot Start PCR Master Mix

Catalog number: K9012
Thermo Scientific™

DreamTaq™ Hot Start PCR Master Mix

Catalog number: K9012
Catalog Number
K9012
Unit Size
1000 rxns
Price (USD)
Price: 890.00
Online price: 783.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
200 rxns
Price (USD)
Price: 199.00
Online price: 175.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K90121000 rxns
Price: 890.00
Online price: 783.65
Your price:
-
K9011200 rxns
Price: 199.00
Online price: 175.65
Your price:
-
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Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. A version of this master mix is also available with pre-added density reagent and electrophoresis tracking dyes.

DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

DreamTaq Hot Start PCR Master Mix is formulated to enhance productivity:
• Better reaction outcomes
   --Higher yield of target amplicons from low template amounts
   --Increased reaction specificity due to reliable hot-start technology
   --Wider range of amplicon lengths - routine amplification of genomic DNA fragments up to 6kb
• Enhanced convenience
   --Room temperature reaction set-up
   --Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
   --Simplified workflow and minimized pipetting steps with 2X master mix format

Applications:
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning

More DreamTaq Hot Start DNA Polymerase products >

Specifications

GC-Rich PCR Performance
Low
Polymerase
DreamTaq DNA Polymerase
Reaction Speed
Standard
Product Line
DreamTaq™
Quantity
1000 Reactions
Shipping Condition
Wet or Dry Ice
Hot Start
Built-In Hot Start
Overhang
3'-A
Reaction Format
SuperMix or Master Mix

Contents & Storage

Contains:
• 20 x 1.25 mL DreamTaq Hot Start PCR Master Mix (2X)
• 20 x 1.25 mL Nuclease-free water

Store at -5 to -30°C.

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