TGF beta 1 ELISA Kit, Multispecies

Catalog number: KAC1688

Novex™  Related applications: Protein Assays and Analysis

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Description

The Multispecies Transforming Growth Factor-beta 1 (Multispecies TGF-β1) ELISA research-use-only kit is to be used for the quantitative determination of TGF-β1 in human, mouse, rat, and swine samples (e.g., serum, plasma, cell culture supernatants, etc.) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of TGF-β1.

Performance characteristics
• Sensitivity: <15.6 pg/mL
• Standard curve range: 31.2–2,000 pg/mL
• Sample types: serum, plasma, cell culture supernatants
• Species cross-reactivity: human, mouse, rat, swine
• Sample volume: 200 μL (extracted and diluted)
• Total assay incubation time: 4 hrs

Principle of the method
The Multispecies TGF-β1 kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for TGF-β1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TGF-β1 content, control specimens, and extracted unknowns, are pipetted into these wells, followed by the addition of a biotinylated second antibody. During the first incubation, TGF-β1 antibody binds simultaneously to the immobilized (capture) antibody on one site, and to the solution phase biotinylated antibody on a second site.

After removal of excess detection antibody, streptavidin-peroxidase (enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of TGF-β1 present in the original specimen.

Background information
Transforming growth factor-alpha (TGF-α) and transforming growth factor-beta (TGF-β) have been implicated in diverse physiologic and pathophysiologic functions including immunological, inflammatory, and neoplastic processes. TGF-β is one of the most potent immunoregulatory molecules known. It is a 25,000-dalton homodimeric protein, with three known isoforms in man: TGF-β1, TGF-β2 and TGF-β3. TGF-βs are synthesized and secreted by various transformed and normal cells including lymphocytes and monocytes. Differential expression of the isoforms of TGF-β is controlled both in vivo and in vitro. Selective regulation of expression of the TGF-βs is under the control of several factors, including oncogenes and tumor suppressor genes. In addition to transcriptional control, TGF-βs appear to be regulated post-transcriptionally. Further evidence for the differential expression of TGF-β isoforms comes from studies of osteosarcoma. In osteosarcoma, the expression of TGF-β3, but not TGF-β2 or TGF-β1, is highly correlated with disease progression.*

Each Multispecies TGF-β1 ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See product insert for more information on validation.

Related links
Learn more about ELISA kits
Learn more about other immunoassays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: Tgfb, CED, DPD1, LAP, TGFB, TGFbeta, TGF-BETA-1, TGF-beta1, TGFbeta1, Tgfb, Tgfb-1
Shipping Condition: Wet Ice
Marker: TGF-β1
Gene ID: 7040, 7040, 21803, 59086, 397078
Species: Human
Gene Symbol: TGFB1, Tgfb1
Sensitivity: <15.6 pg/mL
Target Gene: transforming growth factor, beta 1
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 assays
Concentration: 31.2-2000 pg⁄ml
Sample Volume: 200 µl
Protein Family: Growth Factors & Receptors
Incubation Time: 4 hrs
Detection Method: Colorimetric
Research Category: Immunology, Apoptosis
Sample Type (Specific): Plasma, Supernatants, Serum
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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