SuperScript® Plus Direct cDNA Labeling Module (no purification) with Alexa Fluor®-aha-dUTPs

Catalog number: L101504

Invitrogen™  Related applications: Microarray Analysis | Nucleic Acid Labeling & Oligo Synthesis

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The SuperScript® Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript® III Reverse Transcriptase (RT) with the simplicity of direct labeling. In place of an enzyme spike-in, the streamlined protocol incorporates balanced sets of novel, array-optimized, nucleotides conjugated to Alexa Fluor® 555 and 647 dyes. This results in more reproducible array data with higher correlation coefficients than using standalone reagents. The SuperScript® Plus Direct cDNA Labeling System provides:

• Optimized, all-inclusive direct labeling with balanced aha-Alexa Fluor® nucleotides, enabling higher numbers of array positives
• Improved correlation coefficients for more accurate data (Figure 1)
• Low elution purification columns included for ease of use

In the SuperScript® Plus cDNA Labeling method, anchored oligo(dT) anneals to the mRNA template (Figure 2). SuperScript® III RT extends from the priming site, incorporating dUTP labeled with Alexa Fluor® dye directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using low-elution volume purification columns before hybridization to the microarray.
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: Alexa Fluor™ 647, Alexa Fluor™ 555
Product Size: 30 reactions
Final Product: cDNA (Labeled)
Labeling Method: Direct Labeling
Labeling Target: cDNA
Detection Method: Fluorescent
Includes Label or Dye: Yes
Sample Type (General): RNA

Contents & storage

All SuperScript® Direct cDNA Labeling Systems/Modules include SuperScript® III Reverse Transcriptase, anchored Oligo(dT), random primers, control RNA, 5X first-strand buffer, 0.1 M DTT, 10 mM dNTP mix, low-elution purification columns, loading buffer, wash buffer, amber collection tubes, and DEPC-treated water. The SuperScript® Plus Direct cDNA Labeling System/Module additionally includes Alexa Fluor® 555- and 647-aha-dUTP. The "Module" does not include purification components. For purification components, see the "System" (L101506).

Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C. Guaranteed stable for 6 months when properly stored.


Manuals & protocols

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