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Invitrogen™
Lipofectamine™ 3000 Transfection Reagent
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Description
Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection
Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:
| Fibroblast (3T3, COS-7) | Myoblast (C2C12, L6 CRL-1458) |
| Hepatocyte (HepG2, HuH7) | Erythroleukemic cell (K562) |
| Breast carcinoma (MCF7, Hs578T) | Prostate carcinoma (LNCap) |
| Lung carcinoma (A549, NCI-H460) | Osteosarcoma (U-2 OS, Saos-2) |
| Colon carcinoma (Caco2, SW480) | Pancreatic carcinoma (PANC-1) |
| Skin melanoma (SK-MEL-28) |
View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >
Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).
Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.
Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Cleavage efficiency for GeneArt® TALENs and CRISPRs
Lipofectamine® 3000 transfection procedure details
Enhanced transfection efficiency using Lipofectamine® 3000
Lipofectamine® 3000 Transfection Reagent delivers better transfection performance than Lipofectamine® 2000 reagent as demonstrated by a customer (Rui Eduardo Castro, PhD, University of Lisbon). Shown are the results of transfection of undifferentiated and 3-day differentiated C2C12 cells in the presence of serum and antibiotic (100 ng GFP reporter vector was used per well in 24-well plates; analyzed 48 hrs later).
Lipofectamine® 3000 transfection workflow
Specifications
| Cell Type: | Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells |
|---|---|
| Sample Type (Specific): | Plasmid DNA, RNAi Plasmids (shRNA, miR), Synthetic siRNA |
| Classification: | Animal Origin-Free |
| Transfection Technique: | Lipid-Based Transfection |
| Serum Compatible: | Yes |
| Product Line: | Lipofectamine® |
| Product Size: | 1.5 mL |
Contents & storage
Contains one vial of Lipofectamine® 3000 Reagent (1.5 mL) and one vial of P3000TM Enhancer Reagent (1.5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. Store at 2–8°C. Do not freeze.
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