LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit, for flow cytometry

Catalog number:  L34856

 Related applications:

Cell Viability, Proliferation & Function

| Flow Cytometry

| Microbiological Analysis

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The LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types. This kit uses a mixture of two nucleic acid stains - green-fluorescent SYTO™ 9 dye and red-fluorescent propidium iodide - for viability determinations, and a calibrated suspension of microspheres for accurate sample volume measurements.

View additional information about all microbiology assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.


Compatible Cells: Bacteria
For Use With (Equipment): Flow Cytometer
Detection Method: Fluorescent
Format: Tube(s)
Excitation⁄Emission (nm): PI: 535⁄617, SYTO® 9: 485⁄498
Flow Cytometer Laser Lines: 488
Form: Solution
Label or Dye: SYTO® 9, propidium iodide
Number of Reactions: 100
Product Line: BacLight™, LIVE/DEAD®
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial SYTO® 9 nucleic acid stain (200 µL, 3.34 mM in DMSO), 1 vial of propidium iodide (200 µL, 20 mM in DMSO), and 1 vial of a microsphere standard (1.0 mL of 6.0 µm diameter microspheres).

Store in refrigerator (2–8°C) and protect from light.


Manuals & protocols