LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green

Back to top


The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence; injured cells exhibit reduced red and green fluorescence.

View a selection guide for all Cell Viability Assays for Flow Cytometry.
For Research Use Only. Not for use in diagnostic procedures.


Compatible Cells: Eukaryotic Cells,Mammalian Cells
Detection Method: Fluorescent
Excitation⁄Emission (nm): C12 Resazurin: 563⁄587,SYTOX® Green 504⁄523
Flow Cytometer Laser Lines: 488
For Use With (Equipment): Flow Cytometer,Fluorescence Microscope,Microplate Reader
Format: Tube(s)
Label or Dye: C12 Resazurin, SYTOX® Green
Number of Reactions: 1,000
Product Line: LIVE/DEAD®,SYTOX®
Product Size: 1 kit
Shipping Condition: Room Temperature
Solubility: DMSO (Dimethylsulfoxide)

Contents & storage

Contains 5 vials of C12-resazurin (40 µg each vial), 1 vial of SYTOX® green (100 µL, 10 µM solution in DMSO), 1 vial of DMSO (1.5 mL), and 10X phosphate buffer (100 mL).

(Store in freezer (-5 to -30°C) and protect from light.