LIVE/DEAD® Cell Vitality Assay Kit, C12 Resazurin/SYTOX® Green

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Description

The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence; injured cells exhibit reduced red and green fluorescence.

View a selection guide for all Cell Viability Assays for Flow Cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Tube(s)
Label or Dye: C12 Resazurin, SYTOX® Green
Product Size: 1 kit
Compatible Cells: Eukaryotic Cells, Mammalian Cells
Detection Method: Fluorescent
Number of Reactions: 1,000
For Use With (Equipment): Microplate Reader, Flow Cytometer, Fluorescence Microscope
Excitation⁄Emission (nm): SYTOX® Green 504⁄523, C12 Resazurin: 563⁄587
Shipping Condition: Room Temperature

Contents & storage

Contains 5 vials of C12-resazurin (40 µg each vial), 1 vial of SYTOX® green (100 µL, 10 µM solution in DMSO), 1 vial of DMSO (1.5 mL), and 10X phosphate buffer (100 mL).

(Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols

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