The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence; injured cells exhibit reduced red and green fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Mammalian Cells, Eukaryotic Cells
Other Label(s) or Dye(s)
C12 Resazurin: 563⁄587, SYTOX™ Green 504⁄523
For Use With (Equipment)
Contents & Storage
Contains 5 vials of C12-resazurin (40 μg each vial), 1 vial of SYTOX™ green (100 μL, 10 μM solution in DMSO), 1 vial of DMSO (1.5 mL), and 10X phosphate buffer (100 mL). (Store in freezer (-5 to -30°C) and p
Induced Jurkat cells stained with LIVE⁄DEAD® Cell Vitality Assay Kit analyzed by flow cytometry.