Invitrogen™

LysoLight™ Deep Red Antibody Labeling Kits

Catalog number: L36003
Invitrogen™

LysoLight™ Deep Red Antibody Labeling Kits

Catalog number: L36003
LysoLight Deep Red Antibody Labeling Kits provide a powerful tool for visualizing the lysosomal localization and catabolic degradation of antibodies and proteins. These kits utilize a unique fluorophore that is only active upon enzymatic cleavage by the lysosomal specific enzyme, Cathepsin B. Outside of the lysosome, LysoLight Deep Red has little to no observable background fluorescence, and only upon localization to the protease rich environment of the Lysosome, does LysoLight become activated and brightly fluoresce.
 
Catalog Number
L36003
Unit Size
Each
Description
LysoLight™ Deep Red Amine Reactive Dye
Quantity
3 x 100 μg
Price (USD)
Price: 520.00
Your price:
Availability
-
Full specifications
Quantity
ColorDeep Red
DescriptionLysoLight™ Deep Red Amine Reactive Dye
Detection MethodFluorescence
For Use With (Application)Antibody and Protein Labeling
For Use With (Equipment)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product TypeReactive dye
Green FeaturesEnhanced stability
Product LineLysoLight™
Quantity3 x 100 μg
SolubilityDMSO (Dimethylsulfoxide)
Excitation/Emission650/668 nm
Unit SizeEach
Catalog Number
Unit Size
Each
Description
LysoLight™ Deep Red Antibody Labeling Kit
Quantity
3 x 100 μg
Price (USD)
Price: 700.00
Your price:
Availability
-
Full specifications
Quantity
ColorDeep Red
DescriptionLysoLight™ Deep Red Antibody Labeling Kit
Detection MethodFluorescence
For Use With (Application)Antibody and Protein Labeling
For Use With (Equipment)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product TypeLabeling Kit
Green FeaturesEnhanced stability
Product LineLysoLight™
Quantity3 x 100 μg
SolubilityDMSO (Dimethylsulfoxide)
Excitation/Emission650/668 nm
Unit SizeEach
Catalog Number
Unit Size
Each
Description
LysoLight™ Deep Red Antibody Labeling Kit
Quantity
3 x 1 mg
Price (USD)
Price: 750.00
Your price:
Availability
-
Full specifications
Quantity
ColorDeep Red
DescriptionLysoLight™ Deep Red Antibody Labeling Kit
Detection MethodFluorescence
For Use With (Application)Antibody and Protein Labeling
For Use With (Equipment)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product TypeLabeling Kit
Green FeaturesEnhanced stability
Product LineLysoLight™
Quantity3 x 1 mg
SolubilityDMSO (Dimethylsulfoxide)
Excitation/Emission650/668 nm
Unit SizeEach
Catalog Number
Unit Size
Each
Description
LysoLight™ Deep Red Amine Reactive Dye
Quantity
500 μg
Price (USD)
Price: 700.00
Your price:
Availability
-
Full specifications
Quantity
ColorDeep Red
DescriptionLysoLight™ Deep Red Amine Reactive Dye
Detection MethodFluorescence
For Use With (Application)Antibody and Protein Labeling
For Use With (Equipment)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product TypeReactive dye
Green FeaturesEnhanced stability
Product LineLysoLight™
Quantity500 μg
SolubilityDMSO (Dimethylsulfoxide)
Excitation/Emission650/668 nm
Unit SizeEach
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeDescriptionQuantityPrice (USD)AvailabilityQuantity
L36003Full specifications
EachLysoLight™ Deep Red Amine Reactive Dye3 x 100 μg
Price: 520.00
Your price:
-
ColorDeep Red
DescriptionLysoLight™ Deep Red Amine Reactive Dye
Detection MethodFluorescence
For Use With (Application)Antibody and Protein Labeling
For Use With (Equipment)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product TypeReactive dye
Green FeaturesEnhanced stability
Product LineLysoLight™
Quantity3 x 100 μg
SolubilityDMSO (Dimethylsulfoxide)
Excitation/Emission650/668 nm
Unit SizeEach
L36001Full specifications
EachLysoLight™ Deep Red Antibody Labeling Kit3 x 100 μg
Price: 700.00
Your price:
-
ColorDeep Red
DescriptionLysoLight™ Deep Red Antibody Labeling Kit
Detection MethodFluorescence
For Use With (Application)Antibody and Protein Labeling
For Use With (Equipment)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product TypeLabeling Kit
Green FeaturesEnhanced stability
Product LineLysoLight™
Quantity3 x 100 μg
SolubilityDMSO (Dimethylsulfoxide)
Excitation/Emission650/668 nm
Unit SizeEach
L36002Full specifications
EachLysoLight™ Deep Red Antibody Labeling Kit3 x 1 mg
Price: 750.00
Your price:
-
ColorDeep Red
DescriptionLysoLight™ Deep Red Antibody Labeling Kit
Detection MethodFluorescence
For Use With (Application)Antibody and Protein Labeling
For Use With (Equipment)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product TypeLabeling Kit
Green FeaturesEnhanced stability
Product LineLysoLight™
Quantity3 x 1 mg
SolubilityDMSO (Dimethylsulfoxide)
Excitation/Emission650/668 nm
Unit SizeEach
L36004Full specifications
EachLysoLight™ Deep Red Amine Reactive Dye500 μg
Price: 700.00
Your price:
-
ColorDeep Red
DescriptionLysoLight™ Deep Red Amine Reactive Dye
Detection MethodFluorescence
For Use With (Application)Antibody and Protein Labeling
For Use With (Equipment)Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product TypeReactive dye
Green FeaturesEnhanced stability
Product LineLysoLight™
Quantity500 μg
SolubilityDMSO (Dimethylsulfoxide)
Excitation/Emission650/668 nm
Unit SizeEach
Showing 4 of 4

The labeling protocol provided in the kits involves a simple and rapid procedure, ensuring minimal hands-on time and reliable results. The kits include all necessary reagents, including the LysoLight Deep Red dye, buffers, and purification columns, to facilitate the antibody labeling process.

The LysoLight Deep Red SDP ester contained in these kits readily reacts with lysine residues in antibodies or proteins to yield a covalently attached fluorogenic sensor. The spin columns included in the kits are used for purifying the labeled antibody from excess dye with yields of 70–95%. The kits contain sufficient reagents for three labeling reactions of 100 µg or 1 mg of antibody. The user guide describes a general protocol for using the amine-reactive, SDP ester form of the LysoLight Deep Red dye for labeling either 100 µg or 1 mg of IgG.

For Research Use Only.

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