Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311

El kit de tampón de IEF con pH 3-10 Novex® está optimizado para su uso con geles de IEF conMás información

Número de catálogo	Cantidad
LC5317	1 Kit

Número de catálogo LC5317

Precio (MXN)

Cantidad:

1 Kit

El kit de tampón de IEF con pH 3-10 Novex® está optimizado para su uso con geles de IEF con pH 3-10 Novex®. Cada kit incluye LC5300, LC5310 y LC5311.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

TampónTampones de cátodos y ánodos

Concentración20 X

Compatibilidad del gelGeles de IEF Novex™

Línea de productosNovex

Tipo de productoKit de tampones IEF

Cantidad1 Kit

Tipo de gelIEF

Intervalo de pHentre 3 y 10

Unit SizeEach

Preguntas frecuentes

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When running an IEF gel, is it okay to use just arginine, instead of a mix of arginine and lysine, in the cathode buffer? Does it matter whether the D, L or D/L form of arginine is used?

Yes, you can use just arginine (either form of arginine) as long as the arginine free base form is used and not the hydrochloric form. Note that the lysine used to make the mixed buffer should be free base lysine; free acid lysine should not be used as a substitute. The reason for adding basic amino acids to the cathode buffer is to increase the ionic strength of the buffer in order to fortify the basic end.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.