When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?
Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.
If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.
High background is caused by ampholytes remaining in the gel.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.
High background is caused by ampholytes remaining in the gel.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.