Novex™ TBE Running Buffer (5X) - FAQs

View additional product information for Novex™ TBE Running Buffer (5X) - FAQs (LC6675)

2 product FAQs found

My Novex TBE buffer has precipitated out of solution. What can I do?

If a slight turbidity develops, the fine precipitate can be dissolved by autoclaving for 5 minutes at 110°C. Do not autoclave in the container supplied. This treatment has no deleterious effect on the buffering properties of TBE.

Do you have the recipe for the TBE running buffer and the Hi-Density TBE Sample Buffer?

Please see the recipes below:

TBE Running Buffer (5X):
Tris base, 54.0 g
Boric acid, 27.5 g
EDTA (free acid), 2.9 g
Deionized water to 1.0 L

Hi-Density TBE Sample Buffer (5X):
5X TBE Running buffer (Cat. No. LC6675), 2 mL
Ficoll Type 400, 1.5 g
Bromophenol blue, 1 mL of 1% solution
Xylene cyanol 1 mL of 1% solution
Deionized water to 10.0 mL