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Adhesion Human Magnetic 6-Plex Panel for Luminex™ Platform

Catalog number: LHC0016M

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Human Adhesion Magnetic 6-Plex Panel for the Luminex™ platform is designed to simultaneously quantify human ICAM, VCAM, P-selectin, E-selectin, PECAM, and PAI-1 in serum, plasma, and tissue culture supernatant samples. By measuring 6 analytes in one well, the multiplex assay panel helps provide more data from each sample, saving both money and time compared to more traditional asssays. This multiplex panel employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The kit is suitable for use with the Luminex™ 200™, FLEXMAP 3D™, and MAGPIX™ systems.

• Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
• High quality—fully qualified antibodies permit excellent specificity and sensitivity
• Fast and easy protocols—perform your assays and analyze your data typically in less than one day

Luminex™ xMAP™ Technology—Effective and Proven Analytical Tool
The Human Adhesion Magnetic 6-Plex Panel is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. Upon completion of the sandwich immunoassay, magnetic beads must be measured using a Luminex™ detection system (MAGPIX™, 100™/200™, or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

Magnetic Beads Let You Do More with Your Time
Magnetic bead-based assays enable automation, decrease hands-on time, and increase throughput and precision. Using the fast and easy Luminex™ protocols, you can obtain your results in as little as 3.5 hours. This line of assays was carefully designed and tested for maximum sensitivity, range, and correlation. Using the same components as our polystyrene bead assays allows for performance with identical quality and consistency. Standard curves generated with this assay system extend over several orders of magnitude of reagent concentrations, while the sensitivity and quality of the assays are comparable to ELISAs and our polystyrene bead assays, enabling superior performance. A natural sample test comparing polystyrene and magnetic bead assays shows magnetic and polystyrene beads give equivalent results.

More Validation for Confidence in Your Results
The Human Adhesion Magnetic 6-Plex Panel has been validated using the same rigorous criteria that distinguishes Luminex™ assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay.

Learn more about all Invitrogen™ assays for the Luminex™ platform.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: Luminex®
Species: Human
Bead Type: Magnetic
Label or Dye: R-PE
Product Size: 100 tests
Protein Family: Other Proteins
Detection Method: Fluorescent
Research Category: Oncology
For Use With (Equipment): Luminex® Instruments
Shipping Condition: Wet Ice

Contents & storage

Contains premixed antibody coated capture beads, standard, premixed detector antibody, diluents, SAV concentrate, buffers, wash solution, flat bottom plate, complete protocol and lot-specific technical data sheet. Store at 2-8°C.

Documents

Manuals & protocols

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