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IL-6 Human Kit for Luminex™ Platform

Catalog number: LHC0061

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

This singleplex bead kit is specifically designed for the measurement of human IL-6 in serum, plasma, and tissue culture supernatant. This assay may be run alone or in combination with other select Luminex™ singleplex kits. The panel is suitable for use with the Luminex™ 100™/200™ and FLEXMAP 3D™ systems. Buffer reagents needed to complete the reaction are sold separately (Cat. No. LHB0001).
Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
High quality—fully qualified antibodies permit excellent specificity and sensitivity
Fast and easy protocols—perform your assay and analyze your data typically in less than one day

Luminex™ xMAP™ Technology—Effective and Proven Analytical Tool
The IL-6 Human Singleplex Bead Kit is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. Upon completion of the sandwich immunoassay, beads must be measured using a Luminex™ detection system (100™/200™ or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

More Validation for Confidence in Your Results
The IL-6 Human Singleplex Bead Kit has been validated using the same rigorous criterion that distinguishes Luminex™ assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. When benchmarked against the competition, our multiplex products consistently deliver excellent results. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay (see Documents section below). Standard curves (Figure 1 and 2) are generated for each lot of product to assure quantitation consistency.

Learn more about all Invitrogen™ assays for the Luminex™ platform.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Marker: IL-6
System: Luminex®
Gene ID: 3569
Species: Human
Bead Type: Polystyrene
Bead Region: 19
Gene Symbol: IL6
Sensitivity: <3 pg/ml
Target Gene: interleukin 6
Gene Aliases: interferon, beta 2, BSF2, HGF, HSF, IFNB2, IL-6
Label or Dye: R-PE
Product Size: 100 tests
Protein Family: Cytokines & Receptors
Accession Number: NP_000591.1, NM_000600.3
Detection Method: Fluorescent
Research Category: Immunology
Sample Type (Specific): Plasma, Serum, Cell Culture Supernatants
For Use With (Equipment): Luminex® Instruments
Shipping Condition: Wet Ice

Contents & storage

Contains capture antibody coated beads, standard, detector antibody and lot-specific technical data sheet. Store at 2-8°C.

Documents

Manuals & protocols

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