TNF alpha Human Singleplex Kit, Ultrasensitive

Catalog number: LHC3013M

Invitrogen™  Related applications: Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


The Human TNF-α Ultrasensitive Magnetic Bead Kit for the Luminex™ platform is specifically designed for quantification of low levels of human TNF-α in serum, plasma, and tissue culture supernatant samples. By mixing this Ultrasensitive kit with other targets from our Ultrasensitive kit portfolio, one can obtain more data from each sample, saving both money and time compared to more traditional methods (such as ELISA). This kit is recommended to be run with the Human Ultrasensitive Magnetic Buffer Reagent Kit (Cat. No. LHB0003M). This assay employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The assay is suitable for use with the Luminex™ 200™, FLEXMAP 3D™, and MAGPIX™ systems.

• Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
• High quality—fully qualified antibodies produce excellent specificity and sensitivity
• Fast and easy protocols—perform your assays and analyze your data typically in less than one day

Luminex™ xMAP™ technology—effective and proven analytical tool
The Human TNF-α Ultrasensitive Magnetic Bead Kit is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead has a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay. Upon completion of the sandwich immunoassay, magnetic beads must be measured using a Luminex™ detection system (MAGPIX™, 100™/200™, or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

Magnetic beads let you do more with your time
Magnetic bead-based assays enable automation, decrease hands-on time, and increase throughput and precision. Using the fast and easy Luminex™ protocols, you can obtain results in as little as 3.5 hours. This line of assays was carefully designed and tested for maximum sensitivity, range, and correlation. Using the same components as our polystyrene bead-based assays allows for performance with identical quality and consistency. Standard curves generated with this assay system extend over several orders of magnitude of reagent concentration, while the sensitivity and quality of the assays are comparable to ELISAs and our polystyrene bead-based assays, enabling superior performance.

More validation for confidence in your results
The Human TNF-α Ultrasensitive Magnetic Bead Kit has been validated using the same rigorous criteria that distinguishes Luminex™ assays from other commercially available assays. Our fully qualified antibodies help ensure the specificity and sensitivity of our assays. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay.

Learn more about all Invitrogen™ assays for the Luminex™ platform.

For Research Use Only. Not for use in diagnostic procedures.


Assay Range: See product documentation
Assay Sensitivity: See product documentation
Bead Region: See product documentation
Former Identifiers: Novex
Gene Name: tumor necrosis factor
Gene Symbol: TNF
Immunoassay Kit Format: Singleplex Kit
Immunoassay Kit Grade: Ultrasensitive
Protein Name: tumor necrosis factor
Target (Kit Named): TNF alpha
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01375
Detection Method: Fluorescent
For Use With (Equipment): Luminex™ Instruments
Gene Aliases: DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF
Gene ID: 7124
Label or Dye: R-PE
Product Size: 100 tests
System: Luminex™
Shipping Condition: Wet Ice

Contents & storage

Antibody coated capture beads, standard, detector antibody, and lot-specific technical data sheet. Store at 2-8°C.


Manuals & protocols