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Inflammatory Cytokine Mouse Magnetic 4-Plex Panel for Luminex™ Platform

Catalog number: LMC0003M

Invitrogen™  Related applications: Protein Assays and Analysis

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The Inflammatory Cytokine Mouse Magnetic 4-Plex Panel for the Luminex™ platform is specifically designed for quantifying mouse GM-CSF, IL-1β, IL-6, and TNF-α in serum, plasma, and tissue culture medium. By measuring 4 analytes simultaneously, the Luminex™ assay panel helps provide more data from each sample, saving both money and time compared to more traditional systems (such as ELISA) used in research. This assay may be run alone or in combination with other select Luminex™ singleplex kits. The panel employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The panel is suitable for use with the Luminex™ 200™, FLEXMAP 3D™, and MAGPIX™ systems.

Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
High quality—fully qualified antibodies permit excellent specificity and sensitivity
Fast and easy protocols—perform your multiplex assay and analyze your data typically in less than one day

Select Mouse Analyte Panel
The Mouse Inflammatory Cytokine Magnetic 4-Plex Panel for the Luminex™ platform provides reagents for the accurate, reproducible, and sensitive quantitation of mouse proteins including:


Luminex™ xMAP™ Technology—Effective and Proven Analytical Tool
The Mouse Inflammatory Cytokine Magnetic 4-Plex Panel is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. Upon completion of the sandwich immunoassay, magnetic beads must be measured using a Luminex™ detection system (MAGPIX™, 100™/200™, or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

MAGPIX™ System—Brings the Power of Multiplex to Every Lab
The MAGPIX™ system may also be used for sample analysis. This instrument utilizes xMAP™ technology and is built on light-emitting diodes (LEDs) for excitation, a CCD camera for bead and analyte detection and high-speed digital signal processors. The MAGPIX™ system is more compact and robust, providing a very cost effective magnetic bead-based multiplexing tool. Simple, out of the box set-up allows researchers to rapidly perform singleplex or multiplex assays of up to 50 targets in single biological samples. The MAGPIX™ system is easy to use—ideal for both new and experienced users alike.

Magnetic Beads Let You Do More with Your Time
Magnetic bead-based assays use Luminex™ MagPlex magnetic microsphere. All other assay components are the same as the equivalent Luminex™ polystyrene bead-based assays providing identical quality and consistency performance. MagPlex technology takes advantage of magnetic properties to simplify assay wash steps and maximize uniformity of results. Magnetic bead-based assays also enable automation, decrease hands-on time, and increase throughput and precision. Easy to use protocols allow you to get your results in as little as 3.5 hours. In addition, these assays are compatible with both vacuum and magnetic wash stations and work well with the MAGPIX™ system as well as other Luminex™ xMAP™ platforms.

More Validation for Confidence in Your Results
The Mouse Inflammatory Cytokine Magnetic 4-Plex Panel has been validated using the same rigorous criteria that distinguishes Luminex™ assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. When benchmarked against the competition, our multiplex products consistently deliver excellent results. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay (see Documents section below).

Learn more about all Invitrogen™ assays for the Luminex™ platform.

For Research Use Only. Not for use in diagnostic procedures.


Marker: GM-CSF, TNF-α, IL-6, IL-1β
System: Luminex®
Gene ID: 12981, 16176, 16193, 21926
Species: Mouse
Bead Type: Magnetic
Bead Region: See Product Insert
Gene Symbol: TNF, IL6, CSF2, IL1B
Sensitivity: See Product Insert
Label or Dye: R-PE
Product Size: 100 tests
Protein Family: Cytokines & Receptors
Target Protein: IL-1β, TNF-α, GM-CSF, IL-6
Detection Method: Fluorescent
Research Category: Immunology
Sample Type (Specific): Plasma, Supernatants, Serum
For Use With (Equipment): Luminex® Instruments
Shipping Condition: Wet Ice

Contents & storage

Contains premixed antibody coated capture beads, standard, premixed detector antibody, diluents, SAV concentrate, buffers, wash solution, complete protocol and lot-specific technical data sheet. Store at 2-8°C.


Manuals & protocols

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