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Lipofectamine™ MessengerMAX™ Transfection Reagent, 0.75 mL - FAQs

Lipofectamine™ MessengerMAX™ Transfection Reagent, 0.75 mL - FAQs

View additional product information for Lipofectamine™ MessengerMAX™ Transfection Reagent - FAQs (LMRNA003, LMRNA001, LMRNA150, LMRNA008, LMRNA015)

5 product FAQs found

Can I deliver single-stranded oligonucleotides with Lipofectamine MessengerMAX Transfection Reagent?

If you are delivering a single-stranded oligonucleotide on its own, we recommend using Lipofectamine RNAiMAX Transfection Reagent. However, Lipofectamine MessengerMAX Transfection Reagent can be used for genome editing purposes when Cas9 mRNA is co-delivered with a sgRNA and a single-stranded or double-stranded donor template.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How do I use Lipofectamine MessengerMAX Reagent to deliver both my GeneArt Cas9 mRNA and IVT gRNA together?

Lipofectamine MessengerMAX Reagent may be used for both single gRNA delivery or multiplexing (gRNA for multiple targets) purposes with high transfection efficiency when used with GeneArt Cas9 mRNA. For a detailed protocol, please refer to the GeneArt Cas9 mRNA manual (https://www.thermofisher.com/order/catalog/product/A25640?ICID=search-a25640). We recommend using either the Invitrogen GeneArt Genomic Cleavage Detection Kit (Cat. No. A24732) or Invitrogen GeneArt Genomic Cleavage Selection Kit (Cat. No. A27663) for mutant analysis.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Which cell types can be used with Lipofectamine MessengerMAX Transfection Reagent?

Lipofectamine MessengerMAX Reagent demonstrates low toxicity and high transfection efficiency for all cell types (easy or difficult-to-transfect, primary, and stem cells).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

When would I use Lipofectamine MessengerMAX Reagent over other transfection reagents?

Lipofectamine MessengerMAX Regent is an excellent reagent for co-delivery of Invitrogen GeneArt CRISPR Cas9 mRNA with in vitro transcribed gRNA for geneome editing purposes. Lipofectamine MessengerMAX Reagent has the added benefit of flexibly delivering short dsDNA or HDR templates (0.5-1 kb) which can be ordered through the Invitrogen GeneArt Strings services.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the Invitrogen Lipofectamine MessengerMAX Transfection Reagent?

Lipofectamine MessengerMAX Transfection Reagent is an animal origin-free transfection reagent, especially formulated for the delivery of mRNA, small RNA (eg.,CRISPR IVT gRNA, siRNA, or miRNA), and short dsDNA or HDR templates (0.5-1 kb). Lipofectamine MessengerMAX Reagent is an excellent reagent choice for CRISPR-mediated genome editing applications.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.