AuthorsBardelmeijer HA, Lingeman H, de Ruiter C, Underberg WJ
JournalJ Chromatogr A
PubMed ID9646486
'In recent years capillary electrophoresis (CE) has been developed into a versatile separation technique, next to gas and liquid chromatography (LC), well suited for the determination of a wide variety of e.g., pharmaceutical, biomedical and environmental samples. The main advantages of CE over chromatographic separation techniques are its simplicity and ... More
Determination of nitrite in waters by microplate fluorescence spectroscopy and HPLC with fluorescence detection.
AuthorsBüldt A, Karst U
JournalAnal Chem
PubMed ID10450150
'A selective and versatile fluorescence spectroscopic method for the determination of nitrite in waters has been developed. Nitrite reacts in the presence of mineral acids with the nonfluorescent N-methyl-4-hydrazino-7-nitrobenzofurazan forming N-methyl-4-amino-7-nitrobenzofurazan, which can be detected by fluorescence spectroscopy with an excitation maximum at lambda = 468 nm and an emission ... More
4-(N-Methylhydrazino)-7-nitro-2,1,3-benzooxadiazole (MNBDH): A Novel Fluorogenic Peroxidase Substrate The authors would like to thank the Fonds der Chemischen Industrie (Frankfurt/Main) for financial support.
AuthorsMeyer J, Büldt A, Vogel M, Karst U
JournalAngew Chem Int Ed Engl
PubMed ID10777639
Determination of acetaldehyde in tobacco smoke using N-methyl-4-hydrazino-7-nitrobenzofurazan and liquid chromatography/mass spectrometry.
AuthorsZurek G, Büldt A, Karst U
JournalFresenius J Anal Chem
PubMed ID11220327
A liquid chromatography/mass spectrometry (LC/MS) method for the determination of carbonyl compounds based on derivatization with N-methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) has been developed. Atmospheric pressure chemical ionization (APCI) in the positive mode proved the most versatile ionization technique for MNBD-hydrazones. APCI/MS spectra were recorded and the detection limits were determined for [M+H]+, ... More