Invitrogen™

MitoTracker™ Dyes for Mitochondria Labeling

Catalog number: M7513
Invitrogen™

MitoTracker™ Dyes for Mitochondria Labeling

Catalog number: M7513
Rosamine & carbocyanine-based staining dyes MitoTracker Orange CMTMRos, Red CMXRos, Orange CM-H2TMRos, Red CM-H2XRos, Red FM, Green FM, & Deep Red FM enable mitochondria visualization with fluorescent imaging. These mitochondria dyes are specially packaged in vials of 50 μg each to create freshly reconstituted dyes without the impact of freeze-thaw cycles to aid in a simplified sample prep for cell analysis workflow.
 
Catalog Number
M7513
also known as M-7513
Unit Size
20 x 50 µg
Description
MitoTracker™ Red CM-H2Xros
Format
Special packaging
Price (USD)
Price: 478.00
Online price: 461.65
Your price:
Availability
-
Full specifications
Quantity
ColorRed
Excitation Wavelength Range579/599 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Red CM-H2Xros
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
Catalog Number
Unit Size
20 x 50 µg
Description
MitoTracker™ Deep Red FM
Format
Special packaging
Price (USD)
Price: 474.00
Online price: 440.65
Your price:
Availability
-
Full specifications
Quantity
ColorDeep Red
Excitation Wavelength Range644/665 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Deep Red FM
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
Catalog Number
Unit Size
20 x 50 µg
Description
MitoTracker™ Red FM
Format
Special packaging
Price (USD)
Price: 475.00
Online price: 447.65
Your price:
Availability
-
Full specifications
Quantity
ColorRed
Excitation Wavelength Range581/644 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Red FM
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
Catalog Number
M7512
also known as M-7512
Unit Size
20 x 50 µg
Description
MitoTracker™ Red CMXRos
Format
Special packaging
Price (USD)
Price: 480.00
Online price: 461.65
Your price:
Availability
-
Full specifications
Quantity
ColorRed
Excitation Wavelength Range579/599 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Red CMXRos
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
Catalog Number
M7514
also known as M-7514
Unit Size
20 x 50 µg
Description
MitoTracker™ Green FM
Format
Special packaging
Price (USD)
Price: 478.00
Online price: 448.65
Your price:
Availability
-
Full specifications
Quantity
ColorGreen
Excitation Wavelength Range490/516 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Green FM
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
Catalog Number
M7510
also known as M-7510
Unit Size
20 x 50 µg
Description
MitoTracker™ Orange CMTMRos
Format
Special packaging
Price (USD)
Price: 478.00
Online price: 442.65
Your price:
Availability
-
Full specifications
Quantity
ColorOrange
Excitation Wavelength Range554/576 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Orange CMTMRos
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
Catalog Number
M7511
also known as M-7511
Unit Size
20 x 50 µg
Description
MitoTracker™ Orange CM-H2TMRos
Format
Special packaging
Price (USD)
Price: 472.00
Your price:
Availability
-
Full specifications
Quantity
ColorOrange
Excitation Wavelength Range554/576 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Orange CM-H2TMRos
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
Showing 7 of 7
Catalog NumberSpecificationsUnit SizeDescriptionFormatPrice (USD)AvailabilityQuantity
M7513
also known as M-7513
Full specifications
20 x 50 µgMitoTracker™ Red CM-H2XrosSpecial packaging
Price: 478.00
Online price: 461.65
Your price:
-
ColorRed
Excitation Wavelength Range579/599 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Red CM-H2Xros
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
M22426Full specifications
20 x 50 µgMitoTracker™ Deep Red FMSpecial packaging
Price: 474.00
Online price: 440.65
Your price:
-
ColorDeep Red
Excitation Wavelength Range644/665 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Deep Red FM
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
M22425Full specifications
20 x 50 µgMitoTracker™ Red FMSpecial packaging
Price: 475.00
Online price: 447.65
Your price:
-
ColorRed
Excitation Wavelength Range581/644 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Red FM
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
M7512
also known as M-7512
Full specifications
20 x 50 µgMitoTracker™ Red CMXRosSpecial packaging
Price: 480.00
Online price: 461.65
Your price:
-
ColorRed
Excitation Wavelength Range579/599 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Red CMXRos
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
M7514
also known as M-7514
Full specifications
20 x 50 µgMitoTracker™ Green FMSpecial packaging
Price: 478.00
Online price: 448.65
Your price:
-
ColorGreen
Excitation Wavelength Range490/516 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Green FM
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
M7510
also known as M-7510
Full specifications
20 x 50 µgMitoTracker™ Orange CMTMRosSpecial packaging
Price: 478.00
Online price: 442.65
Your price:
-
ColorOrange
Excitation Wavelength Range554/576 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Orange CMTMRos
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
M7511
also known as M-7511
Full specifications
20 x 50 µgMitoTracker™ Orange CM-H2TMRosSpecial packaging
Price: 472.00
Your price:
-
ColorOrange
Excitation Wavelength Range554/576 nm
FormatSpecial packaging
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Quantity20 x 50 μg
Detection MethodFluorescence
DescriptionMitoTracker™ Orange CM-H2TMRos
Product LineMitoTracker™
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationMitochondria/Organelle Stain
Unit Size20 x 50 µg
Showing 7 of 7

Rosamine-based MitoTracker dyes for mitochondria labeling
Cell-permeant MitoTracker probes stain active mitochondria in live cells for labeling and localization in fluorescent cell imaging. Our rosamine-based mitochondrial staining dyes include MitoTracker Orange CMTMRos, a derivative of tetramethylrosamine, and MitoTracker Red CMXRos, a derivative of X-rosamine. MitoTracker Orange CM-H2TMRos and MitoTracker Red CM-H2XRos are reduced, nonfluorescent versions of the rosamine-based MitoTracker dyes that fluoresce upon oxidation. The fluorescent signal from the rosamine-based MitoTracker dyes is retained in the mitochondria even after aldehyde fixation and detergent permeabilization, making these mitochondria dyes flexible for many workflows including applications that require subsequent processing such as immunocytochemistry or in situ hybridization. MitoTrackerRed and Orange are well suited for multicolor labeling experiments because their fluorescence is well resolved from the green fluorescence of other probes.

Carbocyanine-based MitoTracker dyes for mitochondria labeling
Cell-permeant MitoTracker dyes stain active mitochondria in live cells for mitochondrial labeling and localization in fluorescent cell imaging. The carbocyanine-based dyes MitoTracker Green FM and MitoTracker Red FM accumulate in active mitochondria but are not well-retained in mitochondria after aldehyde fixation. The carbocyanine-based MitoTracker Deep Red FM stains active mitochondrial in live cells and is well-retained in mitochondria after aldehyde fixation and subsequent permeabilization with detergents for applications that require subsequent processing such as immunocytochemistry or in situ hybridization. MitoTracker Red FM and MitoTracker Deep Red FM are well suited for multicolor labeling experiments because their red fluorescence is well resolved from the green fluorescence of other probes.

Benefits of using MitoTracker mitochondria labeling dyes
MitoTracker dyes are provided in vials of 50 μg of lyophilized powder ready for reconstitution. To label mitochondria, live cells are simply incubated with the MitoTracker probe of your choice. The mitochondrial staining dyes passively diffuse across the plasma membrane and accumulate in active mitochondria. The MitoTracker dyes are offered in a range of wavelengths and can be used for mitochondrial localization in multicolor experiments.

Conventional fluorescent stains such as tetramethylrosamine and rhodamine 123 are readily sequestered by active mitochondria and are reversible in dynamic membrane potential measurements as they easily wash out of cells upon loss in membrane potential. In contrast, MitoTracker dyes contain a mildly thiol-reactive chloromethyl moiety so that mitochondrial staining is retained if the mitochondrial membrane potential is lost, allowing many of the MitoTracker dyes to be retained during cell fixation.

Throughout the cell life cycle, mitochondria use oxidizable substrates to produce an electrochemical proton gradient across the mitochondrial membrane (whose potential is negative), resulting in ATP production. MitoTracker dyes are ideal probes for mitochondria staining in experiments studying the cell cycle or processes such as apoptosis and other end point assays. MitoTracker dyes are also available for flow cytometry applications (Cat. Nos. M46750, M46751, M46752, and M46753).

Related products for mitochondrial membrane potential
For studying dynamic mitochondria membrane potential specifically, we recommend using JC-1 (cationic carbocyanine dye, Cat. No T3168) or TMRM (tetramethyl rhodamine methyl ester, Cat. No. I34361) dyes. The MitoProbe JC-1 Assay Kit (Cat M34152) contains the JC-1 dye in addition to the potent mitochondrial membrane-potential disrupter, CCCP, which depolarizes the mitochondrial membrane. These reagents can provide compensatory controls to correctly compensate green-to-red fluorescence ratio. TMRM is used for the detection of dynamic measurement of mitochondrial membrane potential.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

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