CD16/CD32 Monoclonal Antibody (FCR4G8) - FAQs

What should I use to block my cells for flow cytometry analysis?

Use serum from the same species as the host species of the secondary antibody for blocking. If the serum is not available, use from 2 to 5% BSA (Fraction V, defatted). If using only a primary antibody, such as directly-labeled mouse primary antibodies, a good blocking reagent is Fc block. CD16 + CD32 Antibody (FRC-4G8) (Cat. No. MFCR004 or MA5-16680) is a low-affinity receptor for the Fc region of immunoglobulin gamma complexes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.