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ULTIMATE MOUSE ORF CLONE - FAQs

View additional product information for ULTIMATE MOUSE ORF CLONE - FAQs (MORF01)

3 product FAQs found

What is the name of the backbone vector that is used for your Ultimate ORF clones? What are its main features and benefits?

The backbone vector that is used is pENTR221, which is a Gateway Entry vector. The insert (an open reading frame that begins with ATG and ends with a TAG stop codon) is flanked by attL1 and attL2 sites. The construct is ready for Gateway LR recombination with a DEST vector containing attR1 and attR2 sites. It is compatible with N-terminal tagged DEST vectors but not with C-terminal tagged DEST vectors. To express the C-terminal tag, the Tag-On-Demand technology would have to be used or the TAG stop codon would have to be mutated. In place of the Tag-On-Demand adenovirus product we sold previously, we offer the vector expressing the tRNA suppressor as a custom service. If you are interested in this service, please e-mail custom.services@lifetech.com.

How does your Ultimate ORF collection differ from your other cDNA clones?

The cDNA clones we offer are Ultimate ORF clones, Full-Length human cDNA clones, MGC clones, and GeneStorm Expression-Tested clones. The Ultimate ORF clones, Full-length human cDNA clones, and the GeneStorm Expression-Ready clones are made in-house, whereas the MGC clones are derived from external sources and distributed by Thermo Fisher Scientific. The Ultimate ORF clones are fully-sequenced and guaranteed to match GenBank sequence information 100% at the amino acid level, whereas the sequence of the other cDNA clones we offer is not guaranteed.

Which clone collection should I pick: Ultimate ORF, Full-length human cDNA, or MGC?

We recommend choosing the Ultimate ORF clones if quality and sequence are your main priority. Further, these clones are provided in a Gateway vector backbone, so it is easy to shuttle the insert into multiple host systems. The Full-length human cDNA clones and MGC clones are only 5´ and 3´ end-sequenced, but Full-length human cDNA clones offer the advantage of being in a Gateway Entry vector backbone. MGC clones are available for human, mouse, and rat species; Ultimate ORF clones are available for human and mouse species and Full-Length human cDNA clones are available only for human species.