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Invitrogen™
Neon™ Transfection System 100 μL Kit
Description
The Neon™ Transfection System 100 µL Kit is designed specifically for use with the Neon™ Transfection System. Use this kit for transfection volumes of 100 µL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100 µL Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Cell Type: | Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells |
|---|---|
| For Use With (Application): | Transfection |
| For Use With (Equipment): | Neon™ Transfection System |
| Sample Type: | Plasmid DNA, Synthetic siRNA |
| Transfection Technique: | Electroporation |
| Format: | Electroporation Pipette Tip |
| Product Type: | Transfection System |
| Quantity: | 25 x 2 reactions |
| Promoter: | CMV |
| Classification: | Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum |
| For Use With: | Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors |
| Sample Loading Volume: | 100µl |
| Serum Compatible: | Yes |
| Shipping Condition: | Room Temperature |
| Starting Material Cell No.: | 2x10^4 - 6 x10^8 cells; Varies by cell type |
| System Type: | Neon™ Transfection System |
| Product Line: | Neon™ |
Contents & storage
The Neon™ Transfection System 100 µL Kit comes with 10 mL resuspension buffer R, 10 mL resuspension buffer T, 75 mL E2 electrolytic buffer, 25 x 100µL reaction tips, 5 electroporation tubes. Refer to the Neon™ Transfection System users manual for protocol instructions. Store at room temperature.
