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Invitrogen™
Neon™ Transfection System 100 µL Kit
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Description
The Neon® Transfection System 100 µL Kit is designed specifically for use with the Neon® Transfection System. Use this kit for transfection volumes of 100 µL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100 µL Neon® Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Cell Type: | Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells |
|---|---|
| Classification: | Animal Origin-Free, Chemically Defined, Low-Protein, Reduced Growth Factors, Reduced Serum, Xeno-Free |
| Compatible Products: | Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors |
| For Use With (Equipment): | Neon® Transfection System |
| Format: | Electroporation Pipette Tip |
| Product Line: | Neon® |
| Product Size: | 25 x 2 reactions |
| Sample Loading Volume: | 100µl |
| Sample Type (Specific): | Plasmid DNA, Synthetic siRNA |
| Serum Compatible: | Yes |
| Shipping Condition: | Room Temperature |
| Starting Material (Cell #): | 2x10^4 - 6 x10^8 cells; Varies by cell type |
| System: | Neon® Transfection System |
| Transfection Technique: | Electroporation |
Contents & storage
The Neon® Transfection System 100 µL Kit comes with 10 mL resuspension buffer R, 10 mL resuspension buffer T, 75 mL E2 electrolytic buffer, 25 x 100µL reaction tips, 5 electroporation tubes. Refer to the Neon® Transfection System users manual for protocol instructions. Store at room temperature.
