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Invitrogen™

Neon™ Transfection System 100 µL Kit

Catalog number:  MPK10025

Related applications:

RNAi

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Description

The Neon® Transfection System 100 µL Kit is designed specifically for use with the Neon® Transfection System. Use this kit for transfection volumes of 100 µL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100 µL Neon® Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Neon® Transfection System
Sample Type: Plasmid DNA, Synthetic siRNA
Format: Electroporation Pipette Tip
Cell Type: Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Classification: Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
For Use With: Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors
Sample Loading Volume: 100µl
Serum Compatible: Yes
Shipping Condition: Room Temperature
Starting Material Cell No.: 2x10^4 - 6 x10^8 cells; Varies by cell type
System Type: Neon® Transfection System
Transfection Technique: Electroporation
Product Line: Neon®

Contents & storage

The Neon® Transfection System 100 µL Kit comes with 10 mL resuspension buffer R, 10 mL resuspension buffer T, 75 mL E2 electrolytic buffer, 25 x 100µL reaction tips, 5 electroporation tubes. Refer to the Neon® Transfection System users manual for protocol instructions. Store at room temperature.