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Invitrogen™

Neon™ Transfection System Starter Pack

Catalog number: MPK5000S
Invitrogen™

Neon™ Transfection System Starter Pack

Catalog number: MPK5000S
Catalog Number
MPK5000S
Unit Size
1 pack
Price (USD)
9,790.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
MPK5000S1 pack
9,790.00
Your Price:
***
Product Overview
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The Neon® Transfection System Starter Pack contains a Neon® Transfection device and two 192 reaction kits (10 µL and 100 µL sample sizes) to get you started in a fast, convenient, and cost effective manner. The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:

Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
Simple—easy to use, with a single reagent kit for all cell types
Versatile—open system allows electroporation parameters to be optimized freely

One-of-a-kind electroporation
Unlike standard cuvette-based electroporation chambers, the Neon® system uses a patented, biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows better maintenance of physiological conditions, resulting in very high cell survival compared to conventional electroporation (see figure).

Plug-n-play out of the box
The transfection process couldn’t be simpler. Draw up the cells and plasmid mix into the Neon® pipette tip, plug into the pipette holder, and press start. Then just pipette the transfected cells into your culture vessel. No more filling and pulling sample from the cuvette and capping and de-capping. The Neon® system uses a simple 3-step transfection procedure. The transfection occurs in the Neon® pipette tip.

Simplified transfection kit for all cell types
Avoid the hassle of determining which proprietary buffer kit will work with your favorite cell type. We have simplified your work with just one transfection kit that is compatible with all cell types. Start your transfection with our optimized protocols for popular cell types, or follow our standard simple optimization procedure for all new cell types.

Perform more transfections with limited or precious cells
Your cells may be limited when you are required to perform neuronal preps or when enriching for homogeneous cell populations via FACS or Dynabeads® enrichment. In these instances, reducing the number of cells required per transfection can allow you to perform more complex experiments with your precious cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Transfection
Product Type
Transfection System Starter Pack
Quantity
1 Pack
Format
Electroporation Pipette Tip
Voltage
100-240 VAC
Classification
Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
Current
3.0-6.0 A
For Use With
Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors
Max. Power
300 W
Purity or Quality Grade
Transfection Grade
Reaction Time
1 sec
Sample Loading Volume
10µl and 100µl
Serum Compatible
Yes
Starting Material Cell No.
2x10^4 - 6 x10^8 cells; Varies by cell type
System Type
Neon™ Transfection System
Max. Voltage
0.5-2.5 kV
Product Line
Neon™
For Use With (Equipment)
Neon™ Transfection System
Sample Type
Plasmid DNA, Synthetic siRNA
Cell Type
Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells

Contents & Storage

The Neon™ Transfection System Starter pack comes with:
• 1 Neon™ Electroporation Device
• 1 Pipette
• 1 Pipette Station
• 1 USB thumbdrive
• 1 Manual
• 1 Power Cord

Also included are one Neon™ Transfection System 100 µl Kit (192 reactions) and one Neon™ Transfection System 10 µl Kit (192 reactions). Store the buffer boxes 4°C and the tips & tubes boxes at room temperature.

Figures

Frequently asked questions (FAQs)

Citations & References