SILAC Protein ID & Quantitation Media Kit, with Lysine and D-MEM-Flex - FAQs

Each kit contains 500 mL media, which can isotopically label approximately 1x10E12 cells, depending on cell type.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Yes, SILAC reagents can be used with other instruments, but you need to carry out the quantitation manually or ask the instrument manufacturer for analysis software packages for automated protein quantitation. Some manufacturers may have new software packages for this application.

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Yes. LC/MS can be used to analyze samples labeled by SILAC. Always consult with your mass spectrometry facility to determine what protocols are most compatible with their instruments.

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Yes. Samples labeled by SILAC can be analyzed by 2-D or in conjunction with 1-D gels to validate results, analyze difficult proteins, and screen for specific proteins that are hard to identify by 1-D SDS-PAGE.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Yes, the kit includes all the components for labeling cells with the heavy lysine. Heavy arginine is sold as a separate item (Cat. No. MS10009) for researchers who want to perform a double-label experiment using heavy lysine and arginine.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.