SILAC Protein ID & Quantitation Media Kit, with Lysine and Advanced D-MEM/F-12-Flex

Catalog number: MS10033

Invitrogen™  Related applications: Protein Mass Spectrometry Analysis | Stem Cell Analysis

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SILAC (Stable Isotopic Labeling using Amino Acids in Cell Culture) Protein ID and Quantitation Kits are complete metabolic protein labeling solutions, enabling identification and quantitation of relative differences in protein abundance as low as 30% between normal and treated cells.
Use SILAC Protein ID and Quantitation Kits to:
• Easily perform differential protein expression analysis in response to stimulus
• Seamlessly integrate cell culture workflows into rigorous analytical platforms
• Reliably analyze post-translational modifications and low abundance proteins
SILAC Protein ID and Quantitation Kits enable detailed quantitative analysis of post-translational modifications, low abundance proteins, phosphoproteins, membrane proteins, gene knockdown experiments and much more. Samples labeled using the SILAC Protein ID and Quantitation Kits can be purified or fractionated with traditional protocols for subsequent analysis by mass spectrometry.

SILAC Protein Identification and Quantitation Flex Media Kits
Standard media formulations often contain components that affect accurate differential protein expression analysis. Invitrogen has expanded its portfolio of SILAC compatible media in a format that enables customization. Starting with a base media, you can customize your optimal formulation by adding FBS, phenol red, and glucose at varying concentrations. New flexible media include:
• SILAC Advanced DMEM/F12-Flex
For Research Use Only. Not for use in diagnostic procedures.


Amino Acid: Lysine
Assay Type: Metabolic Protein Labeling & Mass Spec
Labeling Method: Metabolic Labeling
Product Size: 1 kit
Shipping Condition: Dry Ice, Room Temperature
Target Molecule: Protein

Contents & storage

SILAC Protein ID and Quantitation Kits contain Gibco™ SILAC Media, Dialyzed Fetal Bovine Serum, L-Glutamine, application-appropriate lysis buffers, PiMAC™ resin, and SILAC amino acids. Store lysis buffers, Dialyzed Fetal Bovine Serum, and L-Glutamine at -20°C. Store Gibco™ SILAC Media, SILAC amino acids, and PiMAC™ resin at 4°C. Guaranteed stable for 6 months when properly stored.


Manuals & protocols