SILAC Amino Acids - FAQs

View additional product information for SILAC Amino Acids - FAQs (MS50001)

3 product FAQs found

How many samples can be tested with the SILAC Protein Quantitation kit?

Each kit contains 500 mL media, which can isotopically label approximately 1x10E12 cells, depending on cell type.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Does SILAC work using LC/MS?

Yes. LC/MS can be used to analyze samples labeled by SILAC. Always consult with your mass spectrometry facility to determine what protocols are most compatible with their instruments.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use 2-D gels to analyze proteins from cells labeled by SILAC?

Yes. Samples labeled by SILAC can be analyzed by 2-D or in conjunction with 1-D gels to validate results, analyze difficult proteins, and screen for specific proteins that are hard to identify by 1-D SDS-PAGE.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.