Chemiluminescent reporter gene assays: sensitive detection of the GUS and SEAP gene products.
AuthorsBronstein I, Fortin JJ, Voyta JC, Juo RR, Edwards B, Olesen CE, Lijam N, Kricka LJ,
JournalBiotechniques
PubMed ID7946301
'Chemiluminescent assays are described for the secreted alkaline phosphatase (SEAP) and beta-glucuronidase (GUS) reporter gene products. These assays provide simple, sensitive, non-isotopic alternatives to existing detection methods and are performed in microplate or tube luminometers or in a scintillation counter. The SEAP reporter gene product is secreted from mammalian cells ... More
Chemiluminescence: sensitive detection technology for reporter gene assays.
AuthorsBronstein I, Martin CS, Fortin JJ, Olesen CE, Voyta JC,
JournalClin Chem
PubMed ID8787727
A series of enzyme-activated chemiluminescence-based assays of reporter gene expression, useful in many biomedical applications, has been developed. The chemiluminescence detection systems for beta-galactosidase, beta-glucuronidase (GUS), and secreted placental alkaline phosphatase (SEAP) reporter enzymes are all based on use of 1,2-dioxetane substrates. This detection technology also permits the combined luminescence ... More