NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each
NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each
Citas y referencias (1)
Invitrogen™

NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each

El kit de etiquetado de tejido multicolor NeuroTrace™ contiene 500 mg cada uno de DiI, DiO o DiD mezclados enMás información
Have Questions?
Número de catálogoCantidad
N228841 kit
Número de catálogo N22884
Precio (CLP)
684.981
Each
Añadir al carro de la compra
Cantidad:
1 kit
Precio (CLP)
684.981
Each
Añadir al carro de la compra
El kit de etiquetado de tejido multicolor NeuroTrace™ contiene 500 mg cada uno de DiI, DiO o DiD mezclados en un gel inerte, resistente al agua. La pasta está lista para su uso como se suministra y puede aplicarse directamente a muestras de tejido vivo o fijo con la punta de una aguja. Este método de aplicación mejora la penetración del colorante en las neuronas agrupadas, etiquetando los axones tanto en la superficie como por debajo de ella
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorVerde, Amarillo, Rojo
Método de detecciónFluorescencia
Para utilizar con (equipo)Microscopio de fluorescencia
Tipo de productoKit de etiquetado de tejidos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Localización subcelularMembranas celulares y lípidos
Línea de productosNEUROTRACE
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I am doing retrograde neuronal labeling using the NeuroTrace DiI Paste. I apply a small glob onto the neuron of interest, but it is not adhering very well. What can I do?

This paste, which has DiI C18 uniformly dispersed within a proprietary hydrophobic carrier, is commonly used to dab onto larger areas than smaller areas commonly used with crystals. Any moisture on the area the paste is applied may be the cause for lack of adherence. Apply the paste using a small flat-head spatula or toothpick and keep it in place a bit longer to ensure contact. The area may also be covered with parafilm to ensure contact as well.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (1)

Citations & References
Abstract
Retinoic acid increases phagocytosis of myelin by macrophages.
Authors:
Journal:J Cell Physiol
PubMed ID:33165955