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Citations & References (90)
Invitrogen™
NP-EGTA (o-Nitrophenyl EGTA, Tetrapotassium Salt, cell impermeant
Photolabile chelator that exhibits high selectivity for Ca2+
| Catalog Number | Quantity |
|---|---|
| N6802 also known as N-6802 | 1 mg |
Catalog number N6802
also known as N-6802
Price (USD)
684.00
Each
Quantity:
1 mg
Price (USD)
684.00
Each
NP-caged EGTA, potassium salt is a cell-impermeant photolabile chelator that exhibits a high selectivity for Ca2+ upon UV illumination, its Kd increases from 80 nM to >1 mM. The NP-EGTA salt can be complexed with Ca2+ to generate a caged calcium complex that will rapidly deliver Ca2+ upon photolysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Dye TypeChelator (Non-Fluorescent)
Quantity1 mg
Shipping ConditionRoom Temperature
Absorbance Wavelength260 nm
Product TypeNP-EGTA
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (90)
Citations & References
Abstract
Spatially organised mitochondrial calcium uptake through a novel pathway in chick neurones.
Journal:Cell Calcium
PubMed ID:16338004
'A brief depolarisation of chick sensory neurones evokes a calcium increase in mitochondria that peaks 1-2s after the depolarisation event and then decays over tens of seconds. Peripheral mitochondria take up more calcium than do central ones, even when the cytosolic calcium increase is spatially homogeneous. The calcium influx into
Ion selectivities of the Ca(2+) sensors for exocytosis in rat phaeochromocytoma cells.
Journal:J Physiol
PubMed ID:11410622
'1. The ion selectivities of the Ca(2+) sensors for the two components of exocytosis in rat phaeochromocytoma (PC12) cells were examined by measurement of membrane capacitance and amperometry. The cytosolic concentrations of metal ions were increased by photolysis of caged-Ca(2+) compounds and measured with low-affinity indicators benzothiazole coumarin (BTC) or
Early requirement for alpha-SNAP and NSF in the secretory cascade in chromaffin cells.
Journal:EMBO J
PubMed ID:10369670
'NSF and alpha-SNAP have been shown to be required for SNARE complex disassembly and exocytosis. However, the exact requirement for NSF and alpha-SNAP in vesicular traffic through the secretory pathway remains controversial. We performed a study on the kinetics of exocytosis from bovine chromaffin cells using high time resolution capacitance
Roles of Ca2+ and crossbridge kinetics in determining the maximum rates of Ca2+ activation and relaxation in rat and guinea pig skinned trabeculae.
Journal:Circ Res
PubMed ID:9686757
'We examined the influences of Ca2+ and crossbridge kinetics on the maximum rate of force development during Ca2+ activation of cardiac myofibrils and on the maximum rate of relaxation. Flash photolysis of diazo-2 or nitrophenyl-EGTA was used to produce a sudden decrease or increase, respectively, in [Ca2+] within Triton-skinned trabeculae
Ca2+ entry in CHO cells, after Ca2+ stores depletion, is mediated by arachidonic acid.
Journal:Cell Calcium
PubMed ID:9883283
'Transfected Chinese hamster ovary cells expressing the rat neurotensin receptor (CHO-NTR cells) were used to study the 'Ca2+ stores depletion-Ca2+ entry' coupling which follows stimulation with neurotensin and liberation of inositol 1,4,5-trisphosphate. This coupling could be dissociated in time: the stores were emptied by stimulation with neurotensin in the absence