Rat Fetal Neural Stem Cell Kit

Catalog number:  N7744200

Gibco™  Related applications: Stem Cell Culture

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GIBCO® Rat Fetal Neural Stem Cells provide superior performance in both adherent cell culture in a matrix and suspended neurosphere cultures. The cells are isolated from the cortex of Sprague-Dawley® rats at day E14 of gestation. Each vial contains 2 × 106 viable cells. For optimal growth performance, we recommend culturing and expanding the cells in StemPro® NSC serum-free media.

Features of the Gibco® Rat Fetal Neural Stem Cell Kit include:

• Superior performance in adherent and spheroid cultures
• >75% of the cells retain their undifferentiated phenotype
• Supports differentiation into neurons, astrocytes, and oligodendrocytes
• Kit comes with cells and associated culture reagents

About neural stem cells
Neural Stem Cells (NSC) are self-renewing multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocytes, and astrocytes. Multipotent neural stem cells can be isolated from fetal or adult central nervous system tissue, or derived from embryonic stem cells. In development, neurogenesis takes place in the third to fourth week of gestation and continues in specific regions of the central nervous system. Because of their capacity to generate neurons and glial cells, neural stem cells are a valuable resource, not only for neuroscience, but also for the potential treatment of neurodegenerative disease and neurological disorders. Adult-derived NSCs tend to be regionally restricted and have limited plasticity compared to fetal-derived NSC.
For Research Use Only. Not for use in diagnostic procedures.



Age: Fetal
Cell Passage Number: Passage 0
Cell Type: Stem Cells (Neural)
Classification: Serum-Free
Culture Type: Adherent Cell Culture, Suspension Cell Culture
Donor Attributes: Normal
Media Recommendation: StemPro® NSC SFM
Number of Cells: 2 x 106
Product Line: Gibco®
Product Size: 1 kit
Quantity: 1 kit
Species: Rat

Contents & storage

This kit is shipped with 1 vial of cells and basal medium, growth supplement and growth factors adequate to formulate 500 ml of complete medium. Components require separate shipping and storage requirements. The growth supplement is not sold individually.

Rat Fetal Neural Stem Cells (p⁄n 510084) 1 ml ( ~2 x 10^6 cells; store in liquid nitrogen)

KnockOut™ DMEM⁄F-12 Basal Medium (500 ml; Store in the dark 2 to 8°C)

StemPro® NSC SFM Supplement ( 10 ml; Store frozen at -5 to -20°C)

FGF Basic Recombinant Human and EGF Recombinant Human (10 µg; Store at 2 to 8°C desiccated).


Manuals & protocols

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